RT-PCR analysed the expression of keratocan and lumican.

<p>RT-PCR was used to determine the expression of keratocan (206 bp), lumican (176 bp) with GAPDH (202 bp) as an internal control in keratocytes at day 8 of culture. Keratocytes cultured on the carriers of acellular bovine cornea under SMG (A) or on the carriers in static condition (B) expressed abundant amounts of keratocan transcript. However, keratocan not expressed in cells cultured on plastic with or without VPA and VC (C, D). Lumican and GAPDH were expressed in the cells from all groups.