mTORC1 activation is involved in HGF-mediated VEGF-A production.

<p>(A) OASFs were incubated with HGF for indicated time intervals, mTORC1 and S6K phosphorylation was examined by Western blotting. (B–D) OASFs were pretreated for 30 min with rapamycin (30 nM) followed by treatment with HGF for 24 h, the VEGF-A expression was examined by qPCR, Western blotting, and ELISA. (E) OASFs were transfected with mTORC1 siRNA followed by stimulation with HGF for 24 h, the VEGF-A expression was examined by ELISA. (F) OASFs were pretreated for 30 min with c-Met inhibitor, Ly294002, and Akt inhibitor for 30 min, followed by stimulation with HGF for 30 min, and mTORC1 phosphorylation was determined by Western blotting. Results are expressed as the mean ± S.E. *, <i>p</i><0.05 compared with control; #, <i>p</i><0.05 compared with HGF-treated group.