Anti-CD25 causes intense alterations in the migration of macrophages and dendritic cells to the lungs of A/J mice, but has a minor effect in B10.A mice.

<p>Characterization of macrophages and dendritic cells by flow cytometry in the lung infiltrating leucocytes (LIL) from anti-CD25-treated and untreated A/J and B10.A mice inoculated i.t. with 1×10⁶<i>P. brasiliensis</i> yeast cells. At weeks 2 and 10 after infection lung cell suspensions were obtained and stained as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0051071#s2" target="_blank">Materials and Methods</a>. The acquisition and analysis gates were restricted to macrophages. Macrophages (A), and dendritic cells (B) at weeks 2 and 10 of infection. The data represent the mean ± SEM of the results from 6–8 mice per group and are representative of two independent experiments. * (<i>P</i><0.05), ** (<i>P</i><0.01), and *** (<i>P</i><0.001) compared with IgG treated controls or the susceptible strain.