Cytokine production by spleen and purified CD4⁺ T cells from mice immunized with DnaK+Tul4+GPI.

<p>Mice were immunized via the i.n. route with DnaK+Tul4+GPI on days 0, 14 and 28. Seven days after the last immunization (A) total splenocytes or (B) purified splenic CD4⁺ T cells co-cultured with irradiated splenocytes/APC at different ratios were stimulated with DnaK or Tul4 at various concentrations, as indicated in figures. Culture supernatants were harvested on day 4 and assessed for the levels of IFN-γ, IL-10 and IL-17 by ELISA. Values are expressed as the mean ± SEM and are representative of 2 separate experiments. Values are significantly different at <i>P</i><0.001 (*), <i>P</i><0.01 (**) and <i>P</i><0.05 (***).