TLR2 and TLR4 involve in HMGB1-induced expressions of TNF-α and IL-1β mRNAs in KCs from burn rats.

<p>Expressions of TNF-α and IL-1β mRNAs were measured by Northern blot analysis. Relative mRNA levels were quantified by densitometry and expressed as OD ratio with GAPDH served as internal standards. HMGB1 showed a stimulatory effect on TNF-α and IL-1β mRNA levels in KCs. Pre-incubation of KCs with anti-TLR2 or anti-TLR4 antibody significantly attenuated HMGB1-induced TNF-α and IL-1β mRNA expressions in KCs from burn rats. (A) A representative result. (B) <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0050668#s3" target="_blank">Results</a> of TNF-α mRNA expression from the independent experiments. (C) <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0050668#s3" target="_blank">Results</a> of IL-1β mRNA expression from the independent experiments. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0050668#s3" target="_blank">Results</a> were given as mean±SEM (n = 3). After burn injury, the KCs treated with medium or HMGB1 alone were used as unstimulated or positive control respectively. ** = p<0.01 <i>vs.</i> unstimulated controls, ANOVA; ^## = p<0.01, <i>vs.</i> positive controls, ANOVA.