Gel filtration chromatography of the β subunit.

<p>The purified wild type β subunit was analyzed on a Superdex 200 16/60 gel filtration column and the elution followed by A₂₈₀. Vertical bars indicate the elution position of relevant standards. The elution volume of all the markers used was: dextran blue (2000 kDa) 39.4 ml; apoferritin (443 kDa) 52.3 ml; β-amylase (200 kDa) 59.5 ml; alcohol dehydrogenase (150 kDa) 64.3 ml; bovine serum albumin (66.2 kDa) 71.6 ml; and cytochrome <i>c</i> (12.4 kDa) 93 ml.