Effects of V1801 on NSCLC cells.

<p>(a, b) The cells were treated with gefitinib or V1801 for 48 h, and analyzed by MTT assay. (c, d) The cells were treated with gefitinib or V1801 for indicated time points and assessed by trypan blue exclusion analysis. (e) Soft-agar colony formation assay for NCI-H1975 cells treated with or without V1801 or gefitinib. (f) Quantitation of foci counting. The mean+SD of three independent experiments is shown. (g, h) NCI-H1975 (g) and HCC827 (h) cells were treated with V1801 or gefitinib for 12 h, lysed, and Western blot assay was performed using indicated antibodies.