Adiponectin expression in bgn^−/0 mice.

<p>A) Adiponectin mRNA levels in EWAT of bgn^+/0 and bgn^−/0 mice on LFD and HFD (n = 9–12). *p<0.05, bgn^−/0 vs. bgn^+/0. B) Western blot analysis of protein levels of adiponectin in EWAT in bgn+/0 and bgn−/0 mice fed a high fat diet. Adiponectin levels are normalized to β-actin. Each lane represents one mouse. C) Serum adiponectin levels measured by ELISA (n = 9–12 mice per treatment group), *p<0.05, bgn^−/0 vs. bgn^+/0. For all graphs in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0050554#pone-0050554-g002" target="_blank">figure 2</a>, results are expressed as mean±SE.