Rhodopsin expression in mice with various numbers of rhodopsin genes.

<p>(A) Northern blot analysis of rhodopsin mRNA from various mouse lines. Copy numbers of wild type rhodopsin and P23H-rhodopsin genes are indicated below each lane, where mRho 0 is mRho^−/−, mRho 1 is mRho^+/−, mRho 2 is mRho^+/+, and mRho 0 NHRE 2 is mRho^−/−NHRE^+/+. Quantification of the 18S and 26S rRNAs bands from the agarose gels prior to transfer (shown as a negative below the Northerns) served as loading controls. Six retinas were used to prepare each mRNA sample. Northern images were obtained on a PhosphorImager. Sizes are indicated in kb.