OxLDL-induced ROS overproduction was attenuated by Kv1.5 siRNA, but exaggerated by Kv1.5 overexpression.

<p>Intracellular ROS levels were detected using DCFH-DA staining in HPAECs transfected with Kv1.5 siRNA (10 nM) or infected with adnovirus containing <i>KCNA5</i>, which were treated with oxLDL (150 µg/ml) for further 1 h. (<b>A</b>) & (<b>B</b>). Kv1.5 siRNA significantly attenuated oxLDL-induced endothelial ROS overproduction in HPAECs, as demonstrated by representative images from laser scanning confocal microscopy and bar graph showing fluorescence intensity measured in Multi-Mode Microplate Reader. (<b>C</b>). Bar graph shows that adnoviral <i>KCNA5</i> gene transfer exaggerated oxLDL-induced endothelial ROS overproduction in HPAECs. The values are presented as means ± SEM of 6 independent experiments. * <i>P</i><0.05, ** <i>P</i><0.01 vs. Vehicle control; # <i>P</i><0.05, ## <i>P</i><0.01 vs. Ad-con.