Quantitative autoradiographic analysis of dopamine receptors, DAT and DTBZ densities in the thalamus.

<p>Autoradiograms show total binding of 1.44 nM [³H]SCH23390, 2.50 nM [³H]raclopride, 3.54 nM [³H]<b>WC-10</b>, 2.19 nM [³H]WIN35428, 4.53 nM [³H]DTBZ (A), and nonspecific binding in presence of 1 µM (+) butaclamol (for [³H]SCH23390), 1 µM <i>S</i>(-)-eticlopride (for [³H]raclopride and [³H]<b>WC-10</b>), 1 µM nomifensine (for WIN35428) and 1 µM <i>S</i>(-)-tetrabenazine (for DTBZ) (B) in the thalamus of human brain sections. The adjacent section shows cresyl violet staining to identify related anatomical structures (C). [³H]Microscale standards (ranging from 0 to 36.3 nCi/mg) were also counted (D). Quantitative analysis of dopamine D₁, D₂, and D₃ receptors, DAT and DTBZ densities (fmol/mg) and the dopamine D₂ ∶ D₃ receptor density ratio in human brain are shown in E and F, respectively. Linear correlation analysis of the average dopamine D₁ and D₃ receptor densities in human thalamus is shown in (G). The numbers 1 through 4 designate the following CNS anatomical regions: 1: Postcommissural putamen (PostPu); 2: Postcommissural caudate (PosCd); 3: Thalamus; 4: Internal capsule (IC). #p<0.01 compared to thalamus.