The relationship between the amount of EFE and accumulation of ethylene in the headspace of closed cultures of <i>E. coli</i>.

<p><i>E. coli</i> DH5α harboring the pDF-trc-EFEh vector with P_trc was cultivated in LB medium and induced with various concentrations of added IPTG. (A) EFEh was purified by affinity chromatography and the amount of target protein was quantified using Image Lab software (BioRad) relative to the total protein content and the final OD₆₀₀ of the cultures. (B) The ethylene accumulation in closed <i>E. coli</i> DH5α cultivation vessels is plotted relative to the amount of recombinant EFE (in percentage relative to the amount of EFEh with maximum dose of IPTG) that was present in each vessel. Protein synthesis was induced by the addition of 25 µM or 1 mM IPTG that was added at an optical density of 0.1 (600 nm). Cultures to which no IPTG was added were used as controls. The concentration of IPTG that was added to each sample is shown in both panels.