Effect of Cre-mediated deletion of the Dyt1 gene on vulnerability of dopaminergic neurons to MPTP.

<p>rAAV-Cre was administered unilaterally to Dyt1-loxP mice and C57/B6 control animals. One month after rAAV treatment, the animals were treated with MPTP. Striatal DA levels were estimated by HPLC at 14 days after MPTP injection. Data are normalized to the striatal DA content of saline-only treatment WT group. Data were reported as the mean±SEM. Two way ANOVA was used to compare potential differences between Cre virus injection side and non-injection side of loxp or WT mice receiving MPTP. However, there was no evidence for enhanced vulnerability after rAAV-Cre in Dyt1-loxP animals. The free base form of MPTP was used in this experiment. Group sizes: WT/Saline (n = 9); loxp/MPTP (n = 10); WT/MPTP (n = 7).