Bak and Bax mediate apoptosis in Ras/E1A-transformed MEF cells.

<p>(<b>A</b>) Expression of both anti-apoptotic and pro-apoptotic Bcl-2 proteins in the indicated cell lines were examined by western blot. (<b>B</b>) The indicated MEF cells were cultured in soft agar and representative images are shown. (<b>C</b>) The number of foci shown in (B). Data represent mean±S.D. of three independent measurements. Asterisks (*) indicate <i>P</i><0.05, Student’s unpaired t test. (<b>D</b>) Both untransformed and transformed Bak^−/−Bax^−/− MEF cells were resistant to apoptotic stimuli. The indicated cells were treated with 0.5 µM thapsigargin and 5 µM etoposide, and cell viability was measured 48 hours later. Mean±S.D. of three independent experiments are shown. Asterisks (*) indicate <i>P</i><0.05, “ns” indicates no significance (P>0.05), Student’s unpaired t test.