TRAF6 negatively regulates the expression of the STAT3 target genes.

<p>(A) TRAF6 inhibited the expression of the STAT3 target gene, <i>CRP</i>. The HEK293 cells were transfected with the indicated amounts of TRAF6. After 24 h, the HEK293 cells were stimulated with IFNα for 15 min and the mRNA levels of <i>CRP</i> were examined by real-time PCR. The expression values were normalized to the expression of <i>GAPDH</i>. Data are presented as the means ± S.D. (n = 3). (B) TRAF6 inhibited the expression of <i>ACT</i>. HEK293 cells were transiently transfected with the indicated combinations of pACT luciferase reporter, pRL-TK control TRAF6, STAT3 and JAK2. After 24 h, the cell lysates were collected for luciferase activity measurements. Data are presented as the means ± S.D. (n = 3). These results are representative of three independent experiments. (**<i>P</i><0.01 compared with control samples which are not transfected with TRAF6, Student's t test).