Neutrophil chemotaxis.

<p>Neutrophils were isolated from vitamin D-sufficient (VDS) or –deficient (VDD) mouse bone marrow for chemotaxis measurement and flow cytometry. (<b>A</b>) VDS neutrophil chemotaxis towards BAL fluid collected from VDS and VDD mice 1 day after LPS stimulation; Chemotaxis of neutrophils isolated from VDS and VDD mice towards varying concentrations of (<b>B</b>) KC/CXCL1 (N = 5 per group) and (<b>C</b>) complement C5a (N = 3 per group); (<b>D</b>) Percentage of CXCR2 positive cells in neutrophils isolated from bone marrow of VDS and VDD mice (N = 4 per group); (<b>E</b>) Expression of CXCR2 as determined by mean fluorescent intensity measured with flow cytometry of CXCR2+ cells from bone marrow of VDS and VDD mice (N = 4 per group).