Scratch wound assay.

<p>Monolayer was wounded by manual scratch with a pipette tip. Solutions containing different HA at 0.2% in culture medium with 2.5% of FCS were distributed at Day 0 and cultures were kept at 37°C for 24 hours (Day1). A: control, B: HMW-HA, C: MMW-HA, D: LMW-HA. Histogram above represents width of the wound measured for each wound at D0 and D1. Results are expressed as a ratio of D1 on D0. <b>Wound healing area</b>: Results are expressed as a ratio of D1 on D0 after image analysis; % of wound area represents the ration of wound area at D1/wound area at D0. We show that only MMW-HA induced a significant wound healing compared to control and other HA (***: p<0.001 compared to culture medium, n = 3). The wound healing factor is three times higher compared to control.