The combination of pan Bcl-2 inhibitor AT-101 with aATC immunotherapy induces pro-apoptotic proteins and inhibits the expression of anti-apoptotic proteins.

<p>L3.6pl cells were either pretreated with AT-101 for 24 h or left untreated followed by additional incubation with ATC or aATC for 4 h and before intracellular staining for pBad, Bax and Bcl-X_L. Top panel show histogram overlays representing isotype control, phosphor-Bad, Bax and Bcl-X_L positive cells. Proportion of phosphor-Bad, Bax and Bcl-X_L positive cells and MFI were gated on tumor cells. Bottom panel shows the graphic representation of MFI under the indicated experimental conditions.