Aging of the <i>Fatp1</i><i>^−/−</i> retina.

<p>A. Electroretinography of <i>Fatp1</i>^+/+ (<i>n</i> = 5) and <i>Fatp1^−/−</i> (<i>n</i> = 11) mice, * <i>p</i> = 0.021 for the whole curve. B. Quantification of autofluorescence in young (<i>n</i> = 3 <i>Fatp1</i>^+/+ and <i>n</i> = 4 <i>Fatp1^−/−</i>) and old (<i>n</i> = 6 <i>Fatp1</i>^+/+ and <i>n</i> = 5 <i>Fatp1^−/−</i>) mice. The values are expressed in arbitrary fluorescent units from the machine. For each mouse, the result is obtained by summing up all values between 520 and 640 nm taken every 10 nm. Error bars represent SEM. <i>Fatp1^−/−</i> PRs remains less responsive to light with age. <i>Fatp1^−/−</i> mice do not accumulate more autofluorescence, thus presumably more lipofuscin, than <i>Fatp1</i>^+/+ with age. C. Upper panel: neutral lipid labeling of <i>Fatp1</i>^+/+ (WT) and <i>Fatp1^−/−</i> (KO) retinas. Ch: choroid. RPE: retinal pigment epithelium. POS: PR outer segments. PIS: PR inner segments. ONL: outer nuclear layer. OPL: outer plexiform layer. INL: inner nuclear layer. IPL: inner plexiform layer. GCL: ganglion cell layer. Arrows indicate the BM. Lower panel: positive control at the same magnification (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0050231#s2" target="_blank">Material and methods</a>). Arrows indicate the BM as in the upper panel. The asterisk indicates positive ORO labeling. <i>Fatp1^−/−</i> or <i>Fatp1</i>^+/+ mice showed no Oil red O labeling in the BM.