Identification and location of slow-cycling SVZ-NSCs.

<p>(<b>A–D</b>) Adult wildtype or Mash1 driven EGFP mice were treated with EdU in drinking water for 3 days and rostral sections immunostained for proposed NSCs markers (ID1/GFAP, <b>A,C</b>) to illustrate co-expression with Mcm2, but exclusion of S-phase marker EdU (<b>B</b>) or Mash1-EGFP (<b>C,D</b>). Arrows in full panels indicate examples of typical Mcm2+ NSCs with other marker combinations. Single captions of single channel confocal planes as well as the corresponding merged image are also presented as insets. Dotted lines mark the approximate ventricular space. Full panel flattened confocal z-stacks are of ∼10 µm thickness. Scale bar in <b>D</b>: 15 µm in full panels and 20 µm in insets. (<b>E</b>) Quantification of Mcm2+ cells expressing other proliferative markers (i.e. Ki67 and EdU). (<b>F</b>) Throughout the different rostro-caudal coordinates of the adult SVZ, the percentage of Mcm2+/EdU− NSCs among the entire population of Mcm2+ cycling cells remained constant, suggesting that the distribution of slow cycling cells is identical to the distribution of all cycling cells. (<b>G</b>) Flattened heat map showing the distribution of NSCs throughout the dorsal and lateral walls of the LV (as shown in <b>Fig. 1</b>). Each column represents a section from the rostral to the caudal LV, with 100 µm sized squares positioned along the dorsal (rows above the black line representing the dorso-lateral corner of the LV) and lateral (rows below the black line). Colours code shows the relative densities of Mcm2+/EdU− NSCs (higher values: red; lower values: blue).