Vps45_L117R complements growth phenotypes of vps45Δ cells.

<p>(A) The specific growth rate (μ) and doubling time of wild-type (SF838-9D) and congenic <i>vps45</i>Δ mutant (NOzY1) cells producing either no HA-Vps45 (carrying the vector YEplac195), wild-type HA-Vps45 (<i>VPS45</i>) or HA-Vps45_L117R (L117R) from pCOG070 and pCOG071 respectively (WT and <i>vps45</i>Δ cells producing no HA-Vps45 carried the vector YEplac195) was determined during logarithmic growth in minimal media. Data represent the mean ± SEM of 6 independent sets of cells. §, P<0.05 versus WT; #, P<0.05 versus <i>vps45</i>Δ. (B) Wild-type cells (SF838-9D) harbouring YEPlac195 or pCOG070 (HA-Vps45), and congenic <i>vps45</i>Δ mutant cells (NOzY1) harbouring plasmids YEplac195 (empty vector), pCOG070 (HA-Vps45), pCOG71 (HA-Vps45_L117R) or pCOG072 (HA-Vps45_W244R) were analysed as described for <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0049628#pone-0049628-g003" target="_blank">Figure 3B</a>. (C) All yeast strains in this panel harbour plasmid pCOG054, producing HA-Snc2 in addition to the following plasmids. Wild-type cells (SF838-9D) harbouring YCplac111 (empty vector) and congenic <i>vps45</i>Δ mutant cells (NOzY1) harbouring YCplac111 (empty vector) or pNB706 (HA-Vps45), pNB707 (HA-Vps45_L117R) or pNB708 (HA-Vps45_W244R) were analyzed as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0049628#pone-0049628-g003" target="_blank">Figure 3B</a>.