Sandwich ELISA of selected antibodies.

<p>Yeast displaying eight selected antibodies were used as Ag85 capture reagents, and tested with the same eight antibodies expressed as scFv-Fc fusions detected using fluorescent anti-rabbit secondary antibodies. Individual histograms show binding of the designated scFv-Fc fusion antibodies to individual yeast cells displaying the indicated antibodies in the presence of 100 nM Ag85. Fluorescence (i.e. the amount of scFv-Fc bound per individual yeast cell) is shown on the X axis, while the yeast count at each fluorescence level is shown on the Y axis. Yeast to the right of the vertical line in each plot are fluorescent as a result of a positive sandwich assay.