CD4 T cells augmented apoptosis and Treg cells resisted apoptosis in treated EAE mice.

<p>A. On day 4 after the second treatment, the splenocytes were prepared for apoptosis test. A. The splenocytes gating on CD4⁺ T cells were analyzed for annexinV/PI by flow cytometry. Shown in each panel is 1 of at least 3 experiments with similar results. B. Gating on CD4⁺CD25⁺ Treg cells, the samples were analyzed for apoptosis by flow cytometry. Shown in each panel is 1 of at least 3 experiments with similar results. C. Summary of the percentage of apoptotic and necrotic CD4⁺ T cells and Treg cells. Bar, mean and SD from 3 independent experiments, each using at least three mice per group (n = 3); *, p<0.05 between the indicated pairs.