Viability of HepG2 cells cultured on µS PVA thin films as quantified using Presto Blue viability assay and expressed relative to viability of these cells on tissue culture polystyrene at a matched initial cell seeding density.

<p>The cells were cultured on (A) pristine µS PVA hydrogels; (B) µS PVA thin films equipped with β-Glu; (C) enzyme-free µS PVA hydrogels in the presence of 1 µM SN-38 glucuronide; (D) enzyme-free µS PVA hydrogels, 1 µM SN-38; (E) µS PVA hydrogels in the presence of 1 µM SN-38 glucuronide and β-Glu added to the cell media (solution based enzyme prodrug therapy); (F) SMEPT conditions, i.e. β-Glu equipped µS PVA thin films in the presence of 1 µM SN-38 glucuronide. Presented results (mean ± st.dev.) are average over at least 3 independent experiments, 3 replicates each.