SM-345431 enhances nerve regeneration into the donor cornea.

<p>Peripheral nerves in the cornea of P0-Cre/Floxed-EGFP mice can be observed by GFP fluorescence (A), and immunohistochemisty of a 40 µm-thick frozen section shows the expression of Sema3A in the basal and suprabasal layers of the corneal epithelium (B, red) and GFP-positive major nerve fibers running through the corneal stromal layer under the epithelium (B, Green). Scale bar = 500 µm in A, 100 µm in B. GFP and βIII tubulin double-positive nerve fibers extending into the donor cornea were traced on an image processing software. The SM-345431-treated group showed a more robust network of regenerating nerves (D, F) compared to vehicle control (C, E).