Inhibitory effect of BAY 60-2770 and sodium nitroprusside (SNP) on intracellular Ca²⁺ levels in human washed platelets activated with collagen or thrombin.

<p>Platelets (1.2×10⁸ platelets/ml) loaded with Fluorfort (10 µM) were pre-incubated or not with ODQ (10 µM, 3 min) and subsequently with BAY 60-2770 (0.01–10 µM, 3 min) or SNP (3 µM, 3 min) before addition of either collagen (2 μg/ml; Panels A and B) or thrombin (0.1 U/ml; Panels C and D). Assays were carried out in the presence of Ca²⁺ (1 mM) to yield total influx of Ca²⁺ (Panels A and C) or in Ca²⁺-free medium with EGTA to yield Ca²⁺ mobilization from internal storage sites (Panels B and D). Results represent the mean values ± SEM (n = 4–7 individuals). *p<0.05 compared with control values (in absence of BAY 60-2770 or SNP); #p<0.05 compared with respective values in absence of ODQ. ^†p<0.05 compared with collagen values in panels A and B.