Inhibitory effect of BAY 60-2770 upon PAC-1 mean fluorescence (activated <i>α_IIbβ₃</i>).

<p>Platelet suspension (20 µl; 1.2×10⁸ platelets/ml) was pre-incubated with ODQ (10 µM) and BAY 60-2770 or respective vehicle (1% DMSO). Platelets were incubated with PAC-1 solution (FITCPAC-1; 10 µl of 25 µg/ml) or control antibody solution (FITC Mouse IgM, same dilution of PAC-1 solution). Platelets were activated with either collagen (2 μg/ml) or thrombin (0.1 U/ml). Mean fluorescence was acquired in flow cytometer (FACSCalibur) equipped with a 488 nm wavelength argon laser using the FL1 channel. Mean fluorescence was considered as a parameter to describe binding intensity to FITC labeled PAC-1. Panels A, B and C show, respectively, the fluorescence intensity in non-activated, collagen- and thrombin-activated platelets. Panels D and E show the mean fluorescence ± SEM (n = 3–4 individuals). *p<0.05 compared with untreated platelets. ^#p<0.05 compared with respective values in the absence of ODQ. ^†p<0.05 compared with respective collagen values in panel D.