The effect of S1P on endocytosis of FITC-labeled dextran by XS52 cells and BM-DCs.

<p>Cells were incubated with FITC-labeled dextran in the presence or absence of the indicated S1P concentrations for 15 min. FITC fluorescence intensity of XS52 cells (A) and BM-DCs was analyzed by flow cytometry. Relative endocytosis by XS52 cells (B) and immature BM-DCs (MHC class II low and CD11c positive) (C) was calculated as mean values of FITC-labeled positive cells after subtraction of 4^oC background fluorescence. Data are expressed as the mean ± SEM of results from at least three independent experiments. *P < 0.05 and **P < 0.01 indicate a statistically significant difference vs. control experiments (B,C).