Temperature-sensitive large T antigen-immortalized prostate epithelial cells (LTA^ts-ECs) express markers of prostate epithelial cells.

<p>LTA^ts-ECs were grown at 33°C without (panels a-i) or with 10 nM DHT (panels j-l) and immunostained with anti-CK18, CK5, or AR antibody. Nuclei were stained with Sytox green. The inset in panel j shows AR expression in prostate epithelial cells detected by Western blotting with anti-AR antibody.