Effects of miR-30c on radiation injury of hFOB and CD34+ cells.

<p>(A) Cell death and proliferation potential were measured using the CellTiter 96R AQueous non-radioactive cell proliferation assay (MTS-assay). The quantity of formazan product as measured by the amount of 490 nm absorbance (OD) is directly proportional to the number of living cells in culture. Levels of OD were dramatically lower in pre-miR30-transfected hFOB cells in both irradiated and non-irradiated samples, compared with controls and miR30 inhibitor-transfected samples (p<0.01). Representative expression from 3 independent experiments is shown. (B) Clonogenicity of human hematopoietic progenitor CD34+ cells was quantified in standard semisolid cultures in triplicate. Colonies were counted 14 days later. Results from two experiments showed that transfection of miR30 inhibitor resulted in significant colony number increases in irradiated cells. Means ± SD. **, p <0.01, miR30c inhibitor vs. controls. Non-TD: miRNA-untransfected control.