Apoptosis induction by CX-4945 and CX-5011.

<p>(A) Apoptosis was assessed by evaluation of nucleosomes present in the cytosol, using the Cell Detection Elisa kit (Roche), after 8 h of CEM cell treatment as indicated. Nucleosome enrichment was calculated from the ratio between the signal in treated and untreated cells; reported values are the means ± SE of four independent experiments. (B) Caspase-dependent PARP cleavage was analyzed by WB on 10 µg proteins of lysate from CEM or U2OS cells treated as indicated. Actin WB was used as loading control. Representative WB of three independent experiments are shown. f.l. PARP: full length PARP.