Cell proliferating ability analysis.

<p>(A–B) Hoechst staining shows the cellular structure of the split-thickness skin (A) and epidermal sheet (B). (C–D) Immunohistochemical staining reveals that most basal keratinocytes (single arrows) in the split-thickness skin (C) and epidermal sheet (D) are positive for P63. Cells around the follicular structures (double arrows) are also positive for P63. (E) The percentage of P63 positive keratinocytes in the epidermal sheet is not significantly different from that in the split-thickness skin (<i>p = </i>0.086, n = 5). Scale bars = 100 µm.