Overexpression of survivin inhibited thimerosal-induced apoptosis in C2C12 myoblast cells.

<p><b>A.</b> C2C12-survivin with overexpression of survivin, C2C12-puro with control vector and normal C2C12 cells (Mock) were subjected to western blot analysis with antibody against survivin followed by densitometric quantification. **P<0.01 vs. C2C12-puro <b>B.</b> C2C12-survivin and C2C12-puro were treated with or without thimerosal (250 nM) for 48 h and stained with Annexin V-FITC/propidium iodide followed by flow cytometry. <b>C.</b> After treatment with or without thimerosal, proteins from C2C12-survivin and C2C12-puro were analyzed by immunoblotting with antibodies against survivin, cytochrome c, cleaved caspase-9, cleaved caspase-3 and GAPDH followed by densitometric quantification. Data are means±S.E.M of the values from three independent experiments. **P<0.01 vs. single treatment with 250 nM thimerosal.