Genetic organization of pOENI-1 and comparison with related sequences.

<p>A. Genetic organization of plasmid pOENI-1. ORFs are represented by numbered arrows and identified by corresponding protein tags (see also <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0049082#pone-0049082-t003" target="_blank">Table 3</a>). B. Sequence comparison of pOENI-1 and related plasmids p1 (CP000424) and pH10 (CP002430). ORFs “c, d” (purple arrows) share 99% similarity with ORFs of pOENI-1v2. C. Portions of chromosomes in <i>O. oeni</i> ATCC BAA 1163 and <i>O. oeni</i> PSU1. The gene OEOE_0812 in <i>O. oeni</i> PSU1 (green arrow) is disrupted in <i>O. oeni</i> ATCC BAA 1163 by an 10 genes insert comprising four genes conserved in pOENI-1 (red arrows) and six genes unrelated to pOENI-1 (pink arrows). The insert is bordered by an 8-bp repeated sequence (dark triangles). D. Genetic organization of pOENI-1v2. ORFs numbered from 1 to 20 share more than 99% nucleotide sequence similarity with corresponding ORFs in pOENI-1. ORFs shaded in purple are not detected in pOENI-1 and code for transposases (a, e, f,), hypothetical proteins (b, c) and a recombinase (d). Pseudogenes are symbolized by arrowheads containing the symbol ψ. Regions of sequence similarity are indicated in percentages and shaded in blue. ori: putative origin of replication.