Specific inhibition by PMSF of the proteolytic activities present in honey_MT1 extracts.

<p>2-D zymography of the honey extracts, in the absence (control, left panels) and in the presence (right panels) of 1.5 mM PMSF, inhibitor of serine proteases. Gels: 1 (<i>orange</i>), 2 (<i>eucalyptus</i>), 3 (<i>chestnut</i>) and 4 (<i>sulla</i>).Only the regions of the gels with isoelectric point between pH 3.5–9.5 and molecular mass between 17–25 kDa are shown.