Down-regulation of miR-574-5p abrogated the TLR9 signaling enhanced tumor progression.

<p>(A) 95D cells were transfected with miR-574-5p inhibitor for 48 h and then assayed for their expression of miR-574-5p. (B) 95D cells were transfected with miR-574-5p inhibitor or the control respectively, and then stimulated with 10 µg/ml CpG ODNs for the indicated time. Error bars indicated standard deviation of triplicate measurements form three independent experiments. (C) Enhanced activity of miR-574-5p-regulated reporter was achieved by infection of 95D cells with the miR-574-5p sponge. (D and E) Groups of eight nude mice were challenged with 2×10⁶ of 95D cells which were stably transfected with miR-574-5p sponge vector or the control vector. Five days later, the tumor bearing mice were injected in situ with 100 µg of CpG ODNs at 7 day intervals. The tumor size and the survival time of tumor bearing mice were determined. Each bar represents the means (±SD) from eight nude mice in each group. *p<0.05.