<i>Fgf10</i> overexpression during homeostasis changes gastric gland histology but not epithelial proliferation in glandular stomach.

<p>(A–B) Hematoxylin and eosin staining of sections of adult glandular stomach in control littermate (<i>R26^rtTA/+; tet(O)Fgf10/+</i> without dox) (A) and mutant <i>Fgf10</i> overexpressing mice (<i>R26^rtTA/+; tet(O)Fgf10/+</i> with dox) (B). There is a visible decrease in the number of parietal cells and an obvious shift of the mucous neck cells from the luminal side towards the gastric gland base (black arrowheads). (C) qRT-PCR showing a significant increase in Fgf10 expression in the mutants vs controls (*p<0.05). (D-E) PCNA immunostaining of sections of adult glandular stomach in control littermate (<i>R26^rtTA/+; tet(O)Fgf10/+</i> without dox) (D) and mutant <i>Fgf10</i> overexpressing mice (<i>R26^rtTA/+; tet(O)Fgf10/+</i> with dox) (E). (F) Quantification of the percentage of PCNA-positive epithelial cells showed a statistically significant increase in the percentage of PCNA positive epithelial cells indicating increased gastric epithelial proliferation in mutant <i>Fgf10</i> overexpressing mice (<i>R26^rtTA/+; tet(O)Fgf10/+</i> with dox) compared to control littermates (<i>R26^rtTA/+; tet(O)Fgf10/+</i> without dox). Dox was given for 10 days. The scale bar is 50 µm. M = mesenchyme, GE = gastric epithelium, L = lumen.