ChIP analysis of the 5′-flanking region of the <i>REG4</i> gene.

<p>A: Schematic representation of the 5′-flanking region of the <i>REG4</i> gene. The location of 4 consensus CDX2-binding sites in the 5′-flanking region of <i>REG4</i> (sites A, B, C, and D) and PCR primers (<i>REG4</i> Primer 1, 2, 3, 4, 5, and 6) are indicated. B: CDX2 binding to <i>REG4</i> promoter region shown by ChIP. Bulk (input) DNA was prepared as well as DNA isolated from ChIP with anti-CDX2 monoclonal antibody or mouse IgG. qPCRs were performed in triplicate for each sample primer set, and the mean and SD of the three experiments was calculated. C: ChIP analysis of H3K27me3 enrichment in the <i>REG4</i> gene promoter. ChIP enrichment was measured using qPCR. qPCRs were performed in triplicate for each sample primer set, and the mean and SD of the three experiments was calculated. <i>P</i> values were calculated using Student′s t-test. * N.S. = not significant.