Effect of S1 on amyloid burden and Aβ levels in APP/PS1 transgenic mice.

<p>Brain sections were stained with ThS, and the images of the cortex (A, B, C) and hippocampus (D, E, F) of AD transgenic mice treated with vehicle (A, D) or S1 (B, E) and WT mice (C, F) were collected. The amounts of soluble Aβ40 (G), soluble Aβ42 (H) and insoluble Aβ40 and Aβ42 (I) in brain extracts were detected by sandwich ELISA using Aβ40 and Aβ42 immunoassay kits (compared with AD mice control, *, <i>P</i><0.05; #, <i>P</i><0.01). Brain extracts were analyzed by Western-blot using antibodies specific for C83, C99, APP and BACE-1, respectively. β-actin in all extracts was used as a loading control (J). The intensity of C99, C83, APP and BACE-1 immunoblots in (J) was quantified. Densitometry values are expressed as values relative to β-actin (K).