CD69 is upregulated on BMDC upon activation.

<p>CD69^+/+ or CD69^−/− GM-CSF BMDC were treated with LPS at different conditions of time and dose. Subsequently they were stained for CD11c and CD69, and analyzed by flow cytometry. <b>A.</b> Overlay of CD69 histograms of CD69^+/+ untreated cells (dashed line), and CD69^–/− (grey filled) and CD69^+/+ (solid line) cells cultured for 12h with 1 µg/ml of LPS (right). <b>B.</b> Time course of CD69 upregulation in response to 1 µg/ml of LPS. CD69 expression is the difference in MFI between CD69^+/+ and CD69^−/− BMDC. <b>C.</b> CD69^+/+ BMDC were cultured for 24h with growing doses of LPS and with anti-CD69 2.2 (to provide a background staining control) or Isotype control. CD69 surface expression is expressed as the difference in MFI between unblocked and blocked samples. Results are representative of two experiments with similar results. <b>D.</b> and <b>E.</b> CD69^+/+ or CD69^−/− Flt3l-derived BMDC were treated with various CpG doses for 24h and stained for the different DC subset markers and CD69. <b>D.</b> Overlay of the CD69 histograms of 50nM CpG-treated CD69^−/− (grey filled), and untreated (dashed line) or 50nM CpG-treated (black line) CD69^+/+ pDC (CD11c⁺, CD45RA⁺, left) and cDC (CD11c⁺, CD45RA⁻, right). <b>E.</b> CD69^+/+ and CD69^−/− BMDC were cultured with growing doses of CpG for 24h. CD69 surface levels are expressed as the difference in MFI between CD69^+/+ and CD69^−/− BMDC. The results shown are of one out of two similar experiments.