Observation of EGFP-positive AT2s and AT1s in TMX-treated transgenic mice after bleomycin treatment.

<p><b>A–C.</b> Scgb1a1-CreER:ACTB-mT-EGFP transgenic mice were given tamoxifen and then treated with bleomycin. Shown are representative images of lung sections analyzed for EGFP (green), pro-SPC (red) and Scgb1a1 (blue) at 21 days post-treatment. Tomato red is not shown. Higher magnification of boxed area in (B) is shown as (C). Arrows in (C) indicate EGFP-positive AT1-like cells. <b>D–F.</b> Representative images of confocal analysis for EGFP (D), PDPN (E) and merged (F) of lung sectons from TMX-treated transgenic mice at 21 days post-bleomycin treatment. Arrows in (D–F) indicate EGFP and PDPN double-positive AT1s. Scale bars: (A, B) 1000 µm; (C–F) 20 µm.