CD69 targeting on DC does not alter their costimulation and priming capacity.

<p><b>A.</b> Flt3l BMDC were cultured with 50 nM CpG and anti-CD69 2.2 (solid line) or IgG1 control (dashed line) for 24 h. CD86 and CD40 expression levels were determined on pDC (CD11c⁺, CD45RA⁺, left) and cDC (CD11c⁺, CD45RA⁻, right). Result representative of two experiments. <b>B.</b> Purified DC were cultured with OT-II CD4⁺ T cells, in the presence of the indicated OVA doses, 0.03 µM CpG and 10 µg/ml anti-CD69 2.2 or IgG1 isotype control, in duplicate, for 3 days. <b>C.</b> Sorted pDC were cultured with the indicated OVA doses and 10 µg/ml anti-CD69 2.2 or IgG1 isotype control for 1 h. After wash, they were co-cultured with CD4⁺ OT-II T cells for 3 days. <b>D.</b> Sorted cDC were pulsed with the indicated OVA doses for 45 minutes and treated with 0.025 µM CpG and anti-CD69 2.2 or IgG1 control for 18 h. Then, they were cultured with OT-I CD8⁺ T cells for 2 days. In all cases, the number of divided cells within Vα2⁺ CD8⁺ or Vα2⁺ CD4⁺ live cells is represented. Bars represent SD of duplicate cultures.