Denervation induces increased Nedd4-1 protein expression in gastrocnemius muscle.

<p><i>Nedd4-1</i> SMS-KO and littermate <i>Myo^Cre;Nedd4-1^+/+</i> control mice underwent right tibial nerve transection with the left hind limb serving as control. Western blotting (WB) of 50 µg gastrocnemius protein lysates denervated for 1 week (A) and 2 weeks (B) show increased Nedd4-1 protein in the denervated (Den) compared to the contralateral control (Con) muscle of <i>Myo^Cre;Nedd4-1^+/+</i> mice. Nedd4-1 is absent from the <i>Nedd4-1</i> SMS-KO control gastrocnemius, but trace Nedd4-1 becomes evident with denervation. Representative blots are shown (n = 8 to 9 mice/cohort).