<i>Nedd4-1</i> SMS-KO gastrocnemius muscle weights are partially spared from denervation induced atrophy.

<p>(A) Total body weights (left panel) and gastrocnemius muscle weights (right panel) of <i>Nedd4-1</i> SMS-KO and littermate <i>Myo^Cre;Nedd4-1^+/+</i> control mice. <i>Nedd4-1</i> SMS-KO mice are slightly smaller, and their gastrocnemius muscle weigh less, than littermate controls (n = 16 pairs, * p<0.05). (B) There is no difference in the basal gastrocnemius muscle mass between the <i>Nedd4-1</i> SMS-KO and control mice when muscle weight is normalized to body weight p>0.05). (C) <i>Nedd4-1</i> SMS-KO and littermate control mice underwent right tibial nerve transection, denervating the gastrocnemius muscle, with the intact left hind limb serving as internal control. Denervated gastrocnemius muscle weights are normalized to the weight of the contralateral control muscle. Denervated muscle of <i>Nedd4-1</i> SMS-KO (solid line, closed squares) mice demonstrates an attenuated atrophic response weighing significantly more than in <i>Myo^Cre;Nedd4-1^+/+</i> (dashed line, open circles) mice at 1 and 2 weeks post denervation, (*p<0.05, n = 8 or 9 mice/cohort. Data are presented as the mean ± SEM).