The effect of DHA on glycerol release and gene expressions of large yellow croaker adipocytes during lipolysis.

<p>The fully differentiated adipocytes were exposed to growth medium without FBS but supplemented with 0, 50, 100 or 200 µmol/L DHA for 24 h before the determining the glycerol concentration in the medium. The gene expression levels were determined by quantitative Real-Time PCR. Data were analyzed by using 2^−ΔΔCt<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0048069#pone.0048069-Livak1" target="_blank">[87]</a> and are referred to the control treatment (DHA = 0) using β-actin as a control. Data are means ± SEM, n = 3. Different letters indicate significant differences at <i>P</i><0.05. ATGL = adipose triglyceride lipase, FAS = fatty acid synthase, LPL = lipoprotein lipase, PPAR = proliferators-activated receptor α, γ.