<p>(A) EMSA with probe-C in lanes 1–9, and hCMEC/D3 nuclear protein in lanes 2–9. Protein-binding was blocked with a consensus Sp oligonucleotide, a mutated Sp oligonucleotide (Sp-mut.) and a consensus AP2 oligonuceotide (lanes 3–5). Supershifts were attempted with antibodies to Sp3, Sp1, TFIID and NFY (lanes 6–9). The nuclear protein produced five shifted bands, of which three (b,c,d) were reduced by anti-Sp3. (B) EMSA with probe-C in lanes 1–7 and Caco-2 nuclear protein in lanes 2–7. The nuclear proteins produced 4 major shifted bands (f–i). Protein-binding was blocked with consensus oligonucleotides for Sp or AP2 (lanes 3 & 4). Supershifts with specific antibodies (lanes 5–7) indicated that band ‘f’ was produced by Sp1, bands ‘g’ and ‘h’...
<p>Supershift experiments were performed by preincubating the nuclear extract from differentiated TH...
<p><b>Copyright information:</b></p><p>Taken from "A critical role for the Sp1-binding sites in the ...
<p>The labeled probe is incubated with nuclear extract (6 µg) from HEK293 cells without any transfec...
<p><b>Copyright information:</b></p><p>Taken from "Sp1/3 and NF-1 mediate basal transcription of the...
<p><b>A</b> SP2 electrophoretic mobility shift was performed running a biotin-labelled Sp2 consensus...
<p>EMSA was performed to analyze the binding ability of putative transcription binding sites. Nuclea...
<p>EMSA analysis of the Sp1 binding region corresponding to the polymorphisms observed in <i>KIR</i>...
<p>(A) Nucleotide sequence of the three wild-type GC-box oligonucleotides probes are shown with unde...
<p>B10R macrophages were treated with MRPs 8/14 (5, 10, 25 µg/ml) for 1 hr or MRP-stimulated before ...
<p>(<b>A</b>) Overview of oligonucleotides used for EMSA. LKB1 DSE: downstream element, position +35...
<p>Purified Sp1 protein (panel A) or nuclear extract from DU145 cells (panels (B) to (D)) were incub...
<p>EMSA bands were obtained incubating either the Cy5 labeled probes IVS1+1505G (A, lane 1) or SP1/S...
<p>(A) EMSA was performed to determine HIF-1α promoter binding complexes. 3T3-L1 cells were treated ...
<p>Shifted <sup>32</sup>P-probe bands sensitive to competition by the WT cold probe are indicated. I...
<p>(A) ChIP was performed on hCMEC/D3 cells with antibodies to TFIID, Sp3, Sp1 and a control IgG. Th...
<p>Supershift experiments were performed by preincubating the nuclear extract from differentiated TH...
<p><b>Copyright information:</b></p><p>Taken from "A critical role for the Sp1-binding sites in the ...
<p>The labeled probe is incubated with nuclear extract (6 µg) from HEK293 cells without any transfec...
<p><b>Copyright information:</b></p><p>Taken from "Sp1/3 and NF-1 mediate basal transcription of the...
<p><b>A</b> SP2 electrophoretic mobility shift was performed running a biotin-labelled Sp2 consensus...
<p>EMSA was performed to analyze the binding ability of putative transcription binding sites. Nuclea...
<p>EMSA analysis of the Sp1 binding region corresponding to the polymorphisms observed in <i>KIR</i>...
<p>(A) Nucleotide sequence of the three wild-type GC-box oligonucleotides probes are shown with unde...
<p>B10R macrophages were treated with MRPs 8/14 (5, 10, 25 µg/ml) for 1 hr or MRP-stimulated before ...
<p>(<b>A</b>) Overview of oligonucleotides used for EMSA. LKB1 DSE: downstream element, position +35...
<p>Purified Sp1 protein (panel A) or nuclear extract from DU145 cells (panels (B) to (D)) were incub...
<p>EMSA bands were obtained incubating either the Cy5 labeled probes IVS1+1505G (A, lane 1) or SP1/S...
<p>(A) EMSA was performed to determine HIF-1α promoter binding complexes. 3T3-L1 cells were treated ...
<p>Shifted <sup>32</sup>P-probe bands sensitive to competition by the WT cold probe are indicated. I...
<p>(A) ChIP was performed on hCMEC/D3 cells with antibodies to TFIID, Sp3, Sp1 and a control IgG. Th...
<p>Supershift experiments were performed by preincubating the nuclear extract from differentiated TH...
<p><b>Copyright information:</b></p><p>Taken from "A critical role for the Sp1-binding sites in the ...
<p>The labeled probe is incubated with nuclear extract (6 µg) from HEK293 cells without any transfec...