Immunofluorescence detection of cl-SNAP-25 in dorsal horns from mice ipl-injected with BoNT/A. A)

<p>Representative examples of low and high magnification confocal images taken from lumbar (L4/L5) sections of spinal cord in naïve mice, not subjected to CCI, injected (<i>panel b</i>) or not with BoNT/A (<i>panel a</i>; 15 pg/paw). Dorsal horn region considered for high magnification images is shown by IDH square in the low magnification image. Scale bar: 20 µM. <b>B)</b> Representative examples of high magnification images taken form lumbar (L4/L5) sections of spinal cord in CCI mice injected with BoNT/A (15 pg/paw). In these images, GFAP (<i>panel a</i>) and Cd11b (<i>panel d</i>) are protein markers of astrocytes and microglia, respectively. <i>Panels b</i> and <i>e</i> show immunofluorescence detection of cl-SNAP-25, while <i>panels c</i> and <i>f</i> show colocalization of cl-SNAP-25 with astrocytes and microglia, and also staining of nuclei with DAPI. Scale bar: 20 µM.