Inhibition of CaMKKβ blocks baicalin-induced AMPKα and ACC phosphorylation in HeLa cells.

<p>(A) Representative Western blots showing the effect of the CaMKKβ inhibitor STO-609 on baicalin-induced phosphorylation of AMPKα at Thr-172 (pAMPKα) and phosphorylation of ACC at Ser-79 (pACC) as well as total AMPKα, ACC and CaMKKβ protein expression. Cells were treated with 5 µM baicalin for 1 hr or 1 µM ionomycin for 5 min in the absence or presence of 10 µg/mL STO-609. (B) Histogram represents the fold change in the pAMPKα/AMPKα or pACC/ACC ratio from at least three independent experiments. * <i>p</i><0.05 compared to respective untreated control; ^#<i>p</i><0.05 and ^##<i>p</i><0.01 compared to respective treatment group in the absence of STO-609.