Characterization of recombinant NiVs in HMVEC-L cells.

<p>(<b>A</b>) Comparative viral growth curves. HMVEC-L cells were infected with each indicated recombinant NiV in triplicate wells at an MOI = 2 for 2 h before inoculum was removed and replenished with fresh media. At the time points indicated, infected cell supernatants were serially diluted before being used to infect Vero cells to calculate Log₁₀TCID₅₀/mL. Error bars indicate one standard deviation of the mean of triplicate infections. ANOVA with Dunnett's multiple comparison test was used to measure the statistical significance of differences in peak viral titer at 48 hours PI when compared with WT recombinant virus. * p<0.001. (<b>B</b>) Western blot analysis of P gene products from infected HMVEC-L cell lysates. HMVEC-L cells were infected with each indicated recombinant NiV at an MOI = 2 for 2 h before inoculum was replaced with fresh media. At 24 h PI, infected cell lysates were harvested in NET-BSA buffer and run on a 4–12% Bis-Tris SDS gel. Proteins were transferred onto PVDF membranes, blocked in 5% milk with TBS-T, and probed with mouse antipeptide sera against indicated proteins. NiV C protein could not be detected.