Fewer transferred monocytes migrate to the dLN during <i>L. mexicana</i> infection compared to <i>L. major</i>.

<p>Monocytes enriched from CD45.1 C57BL/6 mice were injected into the ear of CD45.2 C57BL/6 mice that were infected for two weeks with either <i>L. major</i> or <i>L. mexicana</i>. Eighteen hours following injection of the monocytes, ears and dLNs were harvested and processed. (<b>A</b>) Absolute number of transferred monocytes (CD11b⁺ CD45.1⁺) in the ear. Cells are previously gated on total, live cells that are singlets. (<b>B</b>) Mean fluorescence intensity (MFI) of Ly6C on transferred cells recovered from the ears of infected mice. Percentage (<b>C</b>) or absolute number (<b>D</b>) of CD45.1⁺ cells in the dLN. These cells are previously gated on live, singlets that are CD11b^hi cells. The results expressed are the mean percentage (± SD for FACS plots) or the mean number of cells (± SE for bar graphs) of 3 mice per group. The results are representative of two experiments. * significantly different (p<0.05) compared to <i>L. major</i> infected mice.